Human RSPO3 Recombinant Protein (RPPB4531)
- SKU:
- RPPB4531
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- RSPO3
- Synonyms:
- R-spondin-3
- Protein with TSP type-1 repeat
- hPWTSR
- Roof plate-specific spondin-3
- Source:
- HEK293 Cells
- Uniprot:
- Q9BXY4
Description
| Product Name: | Human RSPO3 Recombinant Protein |
| Product Code: | RPPB4531 |
| Size: | 20µg |
| Species: | Human |
| Target: | RSPO3 |
| Synonyms: | R-spondin-3, Protein with TSP type-1 repeat, hPWTSR, Roof plate-specific spondin-3, hRspo3, Thrombospondin type-1 domain-containing protein 2, RSPO3, PWTSR, THSD2, THSD2, CRISTIN1. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | RSPO3 was lyophilized from a 0.2µm filtered solution in 20mM PB, and 150mM NaCl pH-7.2. |
| Solubility: | It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS PAGE. |
| Amino Acid Sequence: | QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP SGYYGTRYPD INKCTKCKAD CDTCFNKNFC TKCKSGFYLH LGKCLDNCPE GLEANNHTME CVSIVHCEVS EWNPWSPCTK KGKTCGFKRG TETRVREIIQ HPSAKGNLCP PTNETRKCTV DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH |
R-spondin-3 (RSPO3) belongs to the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein which is widely expressed in many tissues. RSPO3 contains 2 Furin-like repeats which have been found in various eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain. RSPO3 acts as an activator of the beta-catenin signaling cascade, initiating TCF-dependent gene activation.
Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid Gln at position 22 to amino acid Val at position 201, fused to an FC, 6 x His-tag at C-terminus, containing a total of 498 amino acids and having a Mw of 47.9 kDa. The protein migrates at 61kDa on SDS-PAGE. RSPO3 is a truncated protein that lacks amino acid Gln at position 201 to amino acid H at position 272 and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6q22.33 Cellular Component: extracellular space; extracellular region Molecular Function:heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding Biological Process: Wnt receptor signaling pathway through beta-catenin |
| NCBI Summary: | This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BXY4 |
| NCBI GenInfo Identifier: | 74752442 |
| NCBI Gene ID: | 84870 |
| NCBI Accession: | Q9BXY4.1 |
| UniProt Secondary Accession: | Q9BXY4,Q5VTV4, Q96K87, B2RC27, |
| UniProt Related Accession: | Q9BXY4 |
| Molecular Weight: | 33,233 Da |
| NCBI Full Name: | R-spondin-3 |
| NCBI Synonym Full Names: | R-spondin 3 |
| NCBI Official Symbol: | RSPO3 |
| NCBI Official Synonym Symbols: | PWTSR; THSD2; CRISTIN1 |
| NCBI Protein Information: | R-spondin-3; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2 |
| UniProt Protein Name: | R-spondin-3 |
| UniProt Synonym Protein Names: | Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2 |
| Protein Family: | R-spondin |
| UniProt Gene Name: | RSPO3 |
| UniProt Entry Name: | RSPO3_HUMAN |
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