R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling; plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1; whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A; Wnt1; and Wnt7A; suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7; Dvl; and JNK. RSPO3 functions by inducing Sdc4-dependent; clathrin-mediated endocytosis. RSPO3 is a novel; evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.
Product Name:
Human RSPO3 Recombinant Protein (RPES3138)
Product Code:
RPES3138
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
R-spondin-3,RSPO3,Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2, CRISTIN1
Accession:
Q9BXY4-1
Sequence:
Met 1-Val 146
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.2. Immobilized RSPO3-His (146) at 10 µg/mL (100 ?L/well) can bind human RNF43-Fc .The EC50 of human RNF43-Fc is 0.01-0.03µg/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
15.3 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
