Human RSPO1 Recombinant Protein (RPPB4529)

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SKU:
RPPB4529
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
RSPO1
Synonyms:
R-spondin-1
Synonyms:
Cristin-3
Synonyms:
mCristin-3
Synonyms:
Roof plate-specific spondin-1
Source:
Chinese Hamster Ovary Cells (CHO)
Uniprot:
Q9Z132
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Description

Product Name:Human RSPO1 Recombinant Protein
Product Code:RPPB4529
Size:20µg
Species:Human
Target:RSPO1
Synonyms:R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific spondin-1, Rspo1, RSPO, R-spondin 1, R-spondin, Rspondin, CRISTIN3.
Source:Chinese Hamster Ovary Cells (CHO)
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a sterile (0.2µm) filtered solution containing PBS.
Solubility:It is recommended to reconstitute the lyophilized RSPO1in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized RSPO1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
Biological Activity:The ED50, as calculated by the Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a is 24.7ng/ml corresponding to a specific activity of 4.0 x 10^4 units/mg.

R-Spondin-1 (Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 induces the onset of crypt cell proliferation and increases intestinal epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.

R-Spondin-1 Human Recombinant produced in CHO cells is a glycosylated monomer chain containing 233 amino acids and having a total molecular mass of 25.6kDa.RSPO1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Cell development/differentiationCellular Component: extracellular space; extracellular region; nucleusMolecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor bindingBiological Process: Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; response to stimulus; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q9Z132
NCBI GenInfo Identifier:227452351
NCBI Gene ID:192199
NCBI Accession:NP_619624.2
UniProt Secondary Accession:Q9Z132,Q3V1S3
UniProt Related Accession:Q9Z132
Molecular Weight:29,332 Da
NCBI Full Name:R-spondin-1
NCBI Synonym Full Names:R-spondin homolog (Xenopus laevis)
NCBI Official Symbol:Rspo1  
NCBI Official Synonym Symbols:Rspondin; R-spondin  
NCBI Protein Information:R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3
UniProt Protein Name:R-spondin-1
UniProt Synonym Protein Names:Cysteine-rich and single thrombospondin domain-containing protein 3; Cristin-3; mCristin-3; Roof plate-specific spondin-1
Protein Family:R-spondin
UniProt Gene Name:Rspo1  
UniProt Entry Name:RSPO1_MOUSE
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