Human ROMO1 (Reactive oxygen species modulator 1) ELISA Kit
The Human ROMO1 (Reactive Oxygen Species Modulator 1) ELISA Kit is specifically designed for the precise quantification of ROMO1 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and accuracy, this kit guarantees dependable and consistent results, making it a valuable tool for a wide array of research investigations.ROMO1 is a critical regulator of reactive oxygen species (ROS) levels in cells, playing a key role in oxidative stress and redox signaling pathways. Dysregulation of ROMO1 has been implicated in various diseases including cancer, neurodegenerative disorders, and inflammatory conditions, underscoring its importance as a biomarker for understanding pathophysiological mechanisms and developing targeted therapeutic interventions.
With its advanced technology and reliable performance, the Human ROMO1 ELISA Kit from AssayGenie offers researchers a powerful tool for studying ROS modulation and its impact on disease progression, paving the way for innovative discoveries and potential therapeutic interventions.
Product Name:
Human ROMO1 (Reactive oxygen species modulator 1) ELISA Kit
SKU:
HUES03431
Target:
Human ROMO1 (Reactive oxygen species modulator 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ROMO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ROMO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ROMO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ROMO1. You can calculate the concentration of Human ROMO1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
97-111
91-104
Average (%)
105
104
98
1:4
Range (%)
87-102
83-98
87-101
Average (%)
94
90
94
1:8
Range (%)
90-106
84-98
82-97
Average (%)
97
90
89
1:16
Range (%)
85-98
79-92
89-102
Average (%)
92
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.29
4.0
0.49
1.27
3.66
Standard deviation
0.03
0.05
0.19
0.03
0.07
0.11
C V (%)
6.38
3.88
4.75
6.12
5.51
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ROMO1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ROMO1 in samples. No significant cross-reactivity or interference between Human ROMO1 and analogues was observed.
