Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) ELISA Kit
The Human RNase3 (ECP) ELISA Kit is specifically designed for the precise measurement of eosinophil cationic protein (ECP) levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Eosinophil cationic protein (ECP), also known as ribonuclease A3, is a vital protein involved in immune response and inflammation.
Elevated levels of ECP have been associated with conditions such as asthma, allergic reactions, and parasitic infections, making it a valuable biomarker for studying these diseases and developing potential treatments.With easy-to-follow instructions and reliable performance, the Human RNase3 (ECP) ELISA Kit is an indispensable tool for researchers seeking to explore the role of ECP in various health conditions and advancements in medical research.
Product Name:
Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) ELISA Kit
SKU:
HUES02422
Target:
Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RNASE3/ECP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNASE3/ECP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNASE3/ECP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RNASE3/ECP. You can calculate the concentration of Human RNASE3/ECP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
87-101
92-104
Average (%)
97
93
98
1:4
Range (%)
89-102
88-102
89-100
Average (%)
95
93
94
1:8
Range (%)
91-107
81-94
87-98
Average (%)
98
86
93
1:16
Range (%)
92-106
81-91
85-98
Average (%)
100
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
123.3
241.53
1242.96
135.11
256.43
1291.01
Standard deviation
6.55
13.21
64.76
9.09
12.87
52.54
C V (%)
5.31
5.47
5.21
6.73
5.02
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RNASE3/ECP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RNASE3/ECP in samples. No significant cross-reactivity or interference between Human RNASE3/ECP and analogues was observed.
