Human Rhodanese Recombinant Protein (RPPB2245)
- SKU:
- RPPB2245
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- Rhodanese
- Synonyms:
- EC 2811
- TST
- MGC19578
- RDS
- Source:
- Escherichia Coli
- Uniprot:
- Q16762
Description
| Product Name: | Human Rhodanese Recombinant Protein |
| Product Code: | RPPB2245 |
| Size: | 20µg |
| Species: | Human |
| Target: | Rhodanese |
| Synonyms: | EC 2.8.1.1, TST, MGC19578, RDS, Thiosulfate sulfurtransferase, Rhodanese. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The Rhodanese protein solution contains 20mM Tris-HCl, pH-8 and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MVHQVLYRAL VSTKWLAESI RTGKLGPGLR VLDASWYSPG TREARKEYLE RHVPGASFFD IEECRDTASP YEMMLPSEAG FAEYVGRLGI SNHTHVVVYD GEHLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLDRS LLKTYEQVLE NLESKRFQLV DSRSQGRFLG TEPEPDAVGL DSGHIRGAVN MPFMDFLTED GFEKGPEELR ALFQTKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPESRVS QGKSEKA |
Rhodanese is a mitochondrial matrix enzyme that is encoded by the nucleus. Rhodanese is involved in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. Rhodanese catalyzes the chemical reaction of thiosulfate & cyanide to sulfite & thiocyanate (detoxification). Rhodanese is part of the transferase family of proteins. Rhodanese includes two highly conservative domains, identified as rhodanese homology domains. In mammals, the majority of cyanide is converted to thiocyanate. Rhodanese has weak mercaptopyruvate sulfurtransferase activity.
Recombinant Human Rhodanese produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular mass of 35.6 kDa. Rhodanese is fused to a 20 amino acid His-Tag at N-terminus and purified by conventional chromatography techniques.
| UniProt Protein Function: | TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA. |
| UniProt Protein Details: | Protein type:EC 2.8.1.1; Mitochondrial; Transferase Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: extracellular space; mitochondrion Molecular Function:5S rRNA binding Biological Process: cyanate catabolic process; epithelial cell differentiation; rRNA transport |
| NCBI Summary: | This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q16762 |
| NCBI GenInfo Identifier: | 3122965 |
| NCBI Gene ID: | 7263 |
| NCBI Accession: | Q16762.4 |
| UniProt Secondary Accession: | Q16762,Q6IB06, B3KRM1, |
| UniProt Related Accession: | Q16762 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | Thiosulfate sulfurtransferase |
| NCBI Synonym Full Names: | thiosulfate sulfurtransferase |
| NCBI Official Symbol: | TST |
| NCBI Official Synonym Symbols: | RDS |
| NCBI Protein Information: | thiosulfate sulfurtransferase |
| UniProt Protein Name: | Thiosulfate sulfurtransferase |
| UniProt Synonym Protein Names: | Rhodanese |
| Protein Family: | Toxic shock syndrome toxin |
| UniProt Gene Name: | TST |
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