ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains; a transmembrane domain; a tyrosine kinase domain; a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain; heart; kidney; in addition to skeletal muscle; parathyroid; cerebellum; pituitary; spleen; testis and breast. And lower levels in thymus; lung; salivary gland; and pancreas. It specifically binds to and is activated by neuregulins; NRG-2; NRG-3; heparin-binding EGF-like growth factor; betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart; the central nervous system and the mammary gland; gene transcription; cell proliferation; differentiation; migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development; and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system; especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation; induction of milk proteins and lactation.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human/Rhesus HER4/ErbB4 Recombinant Protein (RPES3882)
1. Measured by its ability to bind biotinylated human Fc-NRG1 (isoform Beta1) in a functional ELISA.2. Measured by its ability to bind biotinylated human NRG1 (isoform Beta1) in a functional ELISA.3. Measured by its ability to bind biotinylated human NRG1 (aa 2-246)-Fc in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ERBB4 (NP_005226.1) (Met1-Arg649) was expressed; fused with the Fc region of human IgG1 at the C-terminus.Human and Rhesus ERBB4 sequences are identical.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
96.6 kDa
AP Mol Mass:
117 kDa
Formulation:
Lyophilized from sterile PBS; pH7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
