The Human RETN (Resistin) CLIA Kit is specially designed for the precise measurement of resistin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it perfect for various research purposes.Resistin is a key protein that plays a significant role in metabolic disorders such as obesity, insulin resistance, and type 2 diabetes.
By measuring resistin levels, researchers can gain valuable insights into the development and progression of these conditions, paving the way for potential diagnostic and therapeutic advancements.Overall, the Human RETN (Resistin) CLIA Kit is an invaluable tool for researchers looking to explore the role of resistin in metabolic disorders and its potential implications for human health.
Product Name:
Human RETN (Resistin) CLIA Kit
SKU:
HUES00694
Target:
Human RETN (Resistin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RETN. You can calculate the concentration of Human RETN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
103-119
86-99
Average (%)
108
110
92
1:4
Range (%)
85-100
84-98
91-104
Average (%)
91
91
98
1:8
Range (%)
84-97
92-103
93-106
Average (%)
91
97
99
1:16
Range (%)
86-100
93-107
94-109
Average (%)
92
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.23
66.99
375.61
45.42
64.95
344.7
Standard deviation
5.26
7.31
24.64
4.88
7.2
38.78
C V (%)
12.17
10.91
6.56
10.74
11.09
11.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
