The Human Renin CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of renin levels in human serum and plasma samples. Renin is a key enzyme in the renin-angiotensin system, responsible for regulating blood pressure and fluid balance in the body. This CLIA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for your research needs. By accurately measuring renin levels, researchers can gain valuable insights into cardiovascular health, hypertension, and kidney diseases.
Whether investigating potential therapeutic targets or monitoring disease progression, the Human Renin CLIA Kit is an essential tool for researchers in the fields of cardiology, nephrology, and pharmacology. Trust in its reliability and precision to advance your research and discoveries in the field.
Product Name:
Human REN (Renin) CLIA Kit
SKU:
HUES00112
Target:
Human REN (Renin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REN. You can calculate the concentration of Human REN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
96-110
96-107
Average (%)
94
102
102
1:4
Range (%)
93-106
100-115
84-97
Average (%)
98
108
90
1:8
Range (%)
93-107
89-104
87-99
Average (%)
98
96
94
1:16
Range (%)
92-107
95-107
102-114
Average (%)
99
100
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.42
88.87
416.2
56.63
81.57
380.66
Standard deviation
5.71
6.96
43.33
5.32
8.11
25.69
C V (%)
10.3
7.83
10.41
9.39
9.94
6.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REN in samples. No significant cross-reactivity or interference between Human REN and analogues was observed.
