Human RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B) CLIA Kit
The Human RELB (v-Rel reticuloendotheliosis viral oncogene homolog B) CLIA Kit offered by Assaygenie is a reliable and accurate tool for detecting levels of RELB in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a variety of research applications.RELB is a key transcription factor involved in various cellular processes, including inflammation, immune response, and cancer development.
By measuring RELB levels, researchers can gain valuable insights into the role of this protein in different diseases and potential therapeutic targets.Whether studying inflammatory pathways, immune system regulation, or cancer biology, the Human RELB CLIA Kit from Assaygenie provides a valuable tool for researchers looking to advance their understanding of RELB's impact on human health.
Product Name:
Human RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B) CLIA Kit
SKU:
HUES01265
Target:
Human RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RELB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RELB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RELB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RELB. You can calculate the concentration of Human RELB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
91-109
90-105
Average (%)
110
99
96
1:4
Range (%)
97-111
99-117
85-98
Average (%)
105
107
90
1:8
Range (%)
95-109
100-113
88-102
Average (%)
103
107
93
1:16
Range (%)
87-100
90-105
85-98
Average (%)
93
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.34
233.4
984.17
100.4
250.54
1056.61
Standard deviation
9.97
20.75
85.92
9.13
29.09
95.62
C V (%)
9.29
8.89
8.73
9.09
11.61
9.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RELB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RELB in samples. No significant cross-reactivity or interference between Human RELB and analogues was observed.
