Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit
The Human REG3 Alpha (Regenerating Islet-Derived Protein 3 Alpha) ELISA Kit is specially designed for the precise measurement of REG3 Alpha levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a wide range of research applications.REG3 Alpha is a key protein involved in cell regeneration and plays a vital role in various physiological processes.
It has been implicated in conditions such as pancreatic cancer, inflammatory bowel disease, and diabetes, highlighting its importance as a biomarker for studying and potentially treating these ailments.By utilizing the Human REG3 Alpha ELISA Kit, researchers can gain valuable insights into the role of REG3 Alpha in various biological processes and diseases, paving the way for new therapeutic strategies and advancements in medical research.
Product Name:
Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit
SKU:
HUES02341
Target:
Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REG3α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human REG3α. You can calculate the concentration of Human REG3α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
93-105
94-112
Average (%)
106
99
102
1:4
Range (%)
92-105
81-95
86-99
Average (%)
97
86
92
1:8
Range (%)
88-100
85-97
88-101
Average (%)
95
91
94
1:16
Range (%)
88-101
85-96
82-93
Average (%)
93
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.66
95.2
457.22
41.71
93.94
473.81
Standard deviation
2.34
4.1
19.16
2.17
4.46
18.53
C V (%)
5.12
4.31
4.19
5.2
4.75
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human REG3α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG3α in samples. No significant cross-reactivity or interference between Human REG3α and analogues was observed.
