Human REG1 beta (Regenerating Islet Derived Protein 1 Beta) CLIA Kit
The Human REG1-β (Regenerating Islet-Derived Protein 1 Beta) CLIA Kit is a powerful tool for the accurate quantification of REG1-β levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.REG1-β is a key protein involved in cell regeneration and immune response regulation, making it a valuable biomarker for various diseases and conditions.
Its role in promoting tissue repair and modulating inflammatory processes positions it as a promising target for therapeutic intervention in conditions such as diabetes, inflammatory bowel disease, and autoimmune disorders.The Human REG1-β CLIA Kit from AssayGenie provides researchers with a reliable tool to study the role of REG1-β in health and disease, offering valuable insights for potential therapeutic strategies and personalized medicine approaches.
Product Name:
Human REG1 beta (Regenerating Islet Derived Protein 1 Beta) CLIA Kit
SKU:
HUES00722
Target:
Human REG1 beta (Regenerating Islet Derived Protein 1 Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REG1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REG1β. You can calculate the concentration of Human REG1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
99-111
89-102
Average (%)
92
105
95
1:4
Range (%)
90-105
101-118
87-103
Average (%)
97
108
95
1:8
Range (%)
90-105
96-110
99-112
Average (%)
96
103
105
1:16
Range (%)
86-99
100-116
87-98
Average (%)
93
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
91-106
99
Cell culture media (n=5)
93-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.61
410.74
1494.96
186.88
385.51
1441.87
Standard deviation
18.03
43.29
136.94
18.87
30.76
108.14
C V (%)
9.17
10.54
9.16
10.1
7.98
7.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REG1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG1β in samples. No significant cross-reactivity or interference between Human REG1β and analogues was observed.
