Human RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit
The Human RBP4 (Retinol-Binding Protein 4) Plasma ELISA Kit is designed for the accurate quantification of RBP4 levels in human plasma samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.RBP4 is a key protein involved in the transportation of retinol and is associated with metabolic disorders, such as obesity, insulin resistance, and Type 2 diabetes.
It serves as a potential biomarker for assessing metabolic health and studying the mechanisms underlying these conditions.With its user-friendly protocol and reliable performance, the Human RBP4 Plasma ELISA Kit from AssayGenie provides researchers with a valuable tool for investigating the role of RBP4 in metabolic disorders and exploring potential therapeutic strategies.
Product Name:
Human RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit
SKU:
HUES02591
Target:
Human RBP4 (Retinol Binding Protein 4, Plasma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RBP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RBP4. You can calculate the concentration of Human RBP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
92-109
88-99
Average (%)
101
100
94
1:4
Range (%)
94-108
80-91
84-96
Average (%)
100
86
89
1:8
Range (%)
91-104
80-95
83-97
Average (%)
98
86
89
1:16
Range (%)
90-100
86-97
88-101
Average (%)
95
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.45
12.0
49.93
5.15
12.27
50.52
Standard deviation
0.36
0.62
2.74
0.35
0.64
2.15
C V (%)
6.61
5.17
5.49
6.8
5.22
4.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RBP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RBP4 in samples. No significant cross-reactivity or interference between Human RBP4 and analogues was observed.
