Human RBP3 (Retinol Binding Protein 3, Interstitial) CLIA Kit
The Human RBP3 (Retinol-Binding Protein 3) Interstitial CLIA Kit is a high-quality assay designed for the precise measurement of RBP3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.RBP3 is a critical protein involved in retinol transport and metabolism, playing a key role in maintaining vision and overall eye health.
Abnormalities in RBP3 expression have been linked to various ocular diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.With its reliable performance and user-friendly format, the Human RBP3 Interstitial CLIA Kit is an indispensable tool for researchers and clinicians looking to investigate the role of RBP3 in eye diseases and explore novel treatment strategies.
Product Name:
Human RBP3 (Retinol Binding Protein 3, Interstitial) CLIA Kit
SKU:
HUES00901
Target:
Human RBP3 (Retinol Binding Protein 3, Interstitial)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RBP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RBP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RBP3. You can calculate the concentration of Human RBP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
87-103
99-114
Average (%)
96
95
106
1:4
Range (%)
96-111
102-116
90-104
Average (%)
102
108
96
1:8
Range (%)
98-112
87-100
87-102
Average (%)
106
93
93
1:16
Range (%)
101-117
95-108
92-106
Average (%)
110
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.55
239.14
966.55
100.15
218.15
878.45
Standard deviation
12.15
27.98
58.19
11.7
20.81
77.39
C V (%)
11.85
11.7
6.02
11.68
9.54
8.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RBP3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RBP3 in samples. No significant cross-reactivity or interference between Human RBP3 and analogues was observed.
