Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
The Human Rac1 (Ras-related C3 botulinum toxin substrate 1) ELISA Kit is specifically designed for the accurate measurement of Rac1 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.Rac1 is a key protein involved in cell signaling pathways, regulating processes such as cell growth, migration, and invasion.
Dysregulation of Rac1 has been implicated in numerous diseases, including cancer, cardiovascular disorders, and neurological conditions, making it an important biomarker for studying these diseases and developing potential therapies.Order the Human Rac1 ELISA Kit today and uncover valuable insights into Rac1 activity in human samples, advancing your research and contributing to the understanding of various disease mechanisms.
Product Name:
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
SKU:
HUES02572
Target:
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Rac1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Rac1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Rac1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Rac1. You can calculate the concentration of Human Rac1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
86-100
94-108
Average (%)
104
92
99
1:4
Range (%)
88-104
85-96
86-99
Average (%)
95
90
91
1:8
Range (%)
89-105
83-97
87-101
Average (%)
96
89
92
1:16
Range (%)
92-108
88-102
84-95
Average (%)
100
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
91-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.23
3.77
0.51
1.21
3.86
Standard deviation
0.03
0.07
0.16
0.03
0.06
0.18
C V (%)
5.77
5.69
4.24
5.88
4.96
4.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Rac1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Rac1 in samples. No significant cross-reactivity or interference between Human Rac1 and analogues was observed.
