Human PTRH2 Recombinant Protein (RPPB2230)
- SKU:
- RPPB2230
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PTRH2
- Synonyms:
- Peptidyl-tRNA hydrolase 2
- mitochondrial
- PTH 2
- Bcl-2 inhibitor of transcription 1
- Source:
- Escherichia Coli
- Uniprot:
- Q9Y3E5
Description
| Product Name: | Human PTRH2 Recombinant Protein |
| Product Code: | RPPB2230 |
| Size: | 20µg |
| Species: | Human |
| Target: | PTRH2 |
| Synonyms: | Peptidyl-tRNA hydrolase 2, mitochondrial, PTH 2, Bcl-2 inhibitor of transcription 1, PTRH2, BIT1, PTH2, CGI-147, FLJ32471, PTRH2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PTRH2 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEYKMILVVR NDLKMGKGKV AAQCSHAAVS AYKQIQRRNP EMLKQWEYCG QPKVVVKAPD EETLIALLAH AKMLGLTVSL IQDAGRTQIA PGSQTVLGIG PGPADLIDKV TGHLKLY |
Peptidyl-tRNA hydrolase 2 (PTRH2) is a mitochondrial protein. PTRH2 is released during apoptosis from the mitochondria to the cytoplasm. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death. Natural substrates for PTRH2 may be petidyl-tRNAs which drop off the ribosome during protein synthesis.
PTRH2 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (64-179 a.a.) and having a molecular mass of 14.9kDa. The PTRH2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PTRH2: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. Monomer. Belongs to the PTH2 family. |
| UniProt Protein Details: | Protein type:EC 3.1.1.29; Mitochondrial; Hydrolase Chromosomal Location of Human Ortholog: 17q23.1 Cellular Component: membrane; mitochondrion; cytosol Molecular Function:protein binding; aminoacyl-tRNA hydrolase activity Biological Process: apoptosis; metabolic process Disease: Neurologic, Endocrine, And Pancreatic Disease, Multisystem, Infantile-onset |
| NCBI Summary: | The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
| UniProt Code: | Q9Y3E5 |
| NCBI GenInfo Identifier: | 6686183 |
| NCBI Gene ID: | 51651 |
| NCBI Accession: | Q9Y3E5.1 |
| UniProt Secondary Accession: | Q9Y3E5,Q9NTE5, B3KUY4, |
| UniProt Related Accession: | Q9Y3E5 |
| Molecular Weight: | 179 |
| NCBI Full Name: | Peptidyl-tRNA hydrolase 2, mitochondrial |
| NCBI Synonym Full Names: | peptidyl-tRNA hydrolase 2 |
| NCBI Official Symbol: | PTRH2 |
| NCBI Official Synonym Symbols: | BIT1; PTH2; CFAP37; CGI-147 |
| NCBI Protein Information: | peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37 |
| UniProt Protein Name: | Peptidyl-tRNA hydrolase 2, mitochondrial |
| UniProt Synonym Protein Names: | Bcl-2 inhibitor of transcription 1 |
| UniProt Gene Name: | PTRH2 |
| UniProt Entry Name: | PTH2_HUMAN |
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