Tyrosine-protein phosphatase non-receptor type 2; also known as T-cell protein-tyrosine phosphatase; PTPN2 and PTPT; is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif; which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth; differentiation; mitotic cycle; and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP; which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation; and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.
Product Name:
Human PTPN2/PTPT Recombinant Protein (RPES1933)
Product Code:
RPES1933
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
PTN2,PTPT,TC-PTP,TCELLPTP,TCPTP
Accession:
P17706-1
Sequence:
Met 1-Asn 314
Fusion tag:
Activity:
Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate; R&D Systems; Catalog # ES006.The specific activity is > 30 µmoles/min/mg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PTPN2 (?P17706-1) (Met 1-Asn 314) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
36.8 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from sterile 20mM Tris; 500mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
