Human PTPN1 Recombinant Protein (RPPB2640)
- SKU:
- RPPB2640
- Product type:
- Recombinant Protein
- Size:
- 50ug
- Species:
- Human
- Target:
- PTPN1
- Synonyms:
- Tyrosine-protein phosphatase non-receptor type 1
- EC 31348
- Protein-tyrosine phosphatase 1B
- PTP-1B
- Source:
- Escherichia Coli
- Uniprot:
- P18031
Description
| Product Name: | Human PTPN1 Recombinant Protein |
| Product Code: | RPPB2640 |
| Size: | 50µg |
| Species: | Human |
| Target: | PTPN1 |
| Synonyms: | Tyrosine-protein phosphatase non-receptor type 1, EC 3.1.3.48, Protein-tyrosine phosphatase 1B, PTP-1B, PTPN1, PTP1B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The protein (1mg/ml) contains 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLHQEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLKCAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWPDFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKDPSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPPRPPKRILEPHN |
| Biological Activity: | Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C. |
Protein Tyrosine Phosphatase 1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of INS signaling by dephosphorylating the phosphotryosine residues of INS receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to IFN stimulation.
Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids and having a molecular mass of 37.3 kDa.
| UniProt Protein Function: | PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes. |
| UniProt Protein Details: | Protein type:EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; Phosphatase Chromosomal Location of Human Ortholog: 20q13.1-q13.2 Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol Molecular Function:protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; actin cytoskeleton reorganization; regulation of endocytosis; insulin receptor signaling pathway; regulation of signal transduction; negative regulation of insulin receptor signaling pathway; blood coagulation Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P18031 |
| NCBI GenInfo Identifier: | 131467 |
| NCBI Gene ID: | 5770 |
| NCBI Accession: | P18031.1 |
| UniProt Secondary Accession: | P18031,Q5TGD8, Q9BQV9, Q9NQQ4, |
| UniProt Related Accession: | P18031 |
| Molecular Weight: | 49,967 Da |
| NCBI Full Name: | Tyrosine-protein phosphatase non-receptor type 1 |
| NCBI Synonym Full Names: | protein tyrosine phosphatase, non-receptor type 1 |
| NCBI Official Symbol: | PTPN1 |
| NCBI Official Synonym Symbols: | PTP1B |
| NCBI Protein Information: | tyrosine-protein phosphatase non-receptor type 1; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental |
| UniProt Protein Name: | Tyrosine-protein phosphatase non-receptor type 1 |
| UniProt Synonym Protein Names: | Protein-tyrosine phosphatase 1B; PTP-1B |
| Protein Family: | Tyrosine-protein phosphatase non-receptor |
| UniProt Gene Name: | PTPN1 |
| UniProt Entry Name: | PTN1_HUMAN |
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