PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. It has been recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. PTPMT1 deficiency in mouse embryonic fibroblasts compromises mitochondrial respiration and results in abnormal mitochondrial morphology. Lipid analysis of PTPMT1-deficient fibroblasts reveals an accumulation of PGP along with a concomitant decrease in phosphatidylglycerol. Modulation of mitochondrial ATP synthesis by PTPMT1 suggests a novel approach for the treatment of pancreatic cancers, which represent some of the deadliest forms of human tumors. The gluttony of cancer cells for energy is well established, and with the development of a modulator of expression, one may hope that we could also achieve the synthetic induction of PTPMT1 expression. It would then be expected that this effect would attenuate, if not abolish, the growth of pancreas-derived tumor cells and support the establishment of a novel regimen for pancreatic cancers.
Product Name:
Human PTPMT1 Recombinant Protein (RPES1713)
Product Code:
RPES1713
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
DUSP23,FLJ46081,MOSP,PLIP,PNAS-129
Accession:
Q8WUK0-1
Sequence:
Lys 28-Thr 201
Fusion tag:
N-His
Activity:
Measured by its ability to cleave pNPP.The specific activity is >200 pmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human PTPMT1 isoform 1 (Q8WUK0-1) (Lys 28-Thr 201) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
21.7 kDa
AP Mol Mass:
20 kDa
Formulation:
Lyophilized from sterile PBS, 10% glycerol, 1mM DTT, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
