The Human PT (Prothrombin) ELISA Kit is a reliable and accurate tool for measuring prothrombin levels in human samples. Prothrombin is a key protein involved in the blood clotting cascade, making it essential for maintaining proper hemostasis in the body. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in serum, plasma, and cell culture supernatants. It is an ideal choice for researchers studying coagulation disorders, monitoring patients on anticoagulant therapy, and investigating prothrombin-related diseases such as thrombosis and hemophilia.
By accurately measuring prothrombin levels, researchers can gain valuable insights into the clotting process and develop novel strategies for managing clotting disorders and preventing thrombotic events. Invest in the Human PT ELISA Kit for reliable and informative results in your research projects.
Product Name:
Human PT (Prothrombin) ELISA Kit
SKU:
HUES02760
Target:
Human PT (Prothrombin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
5.63 ng/mL
Detection range:
9.38-600 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PT. You can calculate the concentration of Human PT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
89-103
86-97
Average (%)
96
96
91
1:4
Range (%)
92-107
85-97
84-94
Average (%)
99
91
89
1:8
Range (%)
92-107
82-95
83-97
Average (%)
98
88
90
1:16
Range (%)
86-100
88-98
81-92
Average (%)
93
93
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
28.24
72.85
262.16
30.55
68.88
263.52
Standard deviation
1.52
4.14
14.39
1.96
3.35
13.7
C V (%)
5.38
5.68
5.49
6.42
4.86
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PT in samples. No significant cross-reactivity or interference between Human PT and analogues was observed.
