Human PSMA6 Recombinant Protein (RPPB2201)
- SKU:
- RPPB2201
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PSMA6
- Synonyms:
- Proteasome (prosome
- macropain) subunit alpha type 6
- PROS27
- p27K
- Source:
- Escherichia Coli
- Uniprot:
- P60900
Description
| Product Name: | Human PSMA6 Recombinant Protein |
| Product Code: | RPPB2201 |
| Size: | 10µg |
| Species: | Human |
| Target: | PSMA6 |
| Synonyms: | Proteasome (prosome, macropain) subunit alpha type 6, PROS27, p27K, IOTA, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, 27 kDa prosomal protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PSMA6 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTVTHLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD |
PSMA6 belongs to the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with an extremely organized ring-shaped 20S core structure. The core structure consists of 4 rings of 28 non-identical subunits; 2 rings consist of 7 alpha subunits and 2 rings consist of 7 beta subunits. PSMA6 is spread all over eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent procedure in a non-lysosomal pathway.
PSMA6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 270 amino acids (1-246 a.a.) and having a molecular mass of 29.9kDa.PSMA6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMA6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Belongs to the peptidase T1A family. |
| UniProt Protein Details: | Protein type:Proteasome complex; EC 3.4.25.1; Protease Chromosomal Location of Human Ortholog: 14q13 Cellular Component: proteasome complex; nucleoplasm; polysome; proteasome core complex; sarcomere; nuclear matrix; myofibril; cytoplasm; nucleus; cytosol Molecular Function:threonine endopeptidase activity; NF-kappaB binding; protein binding; RNA binding; endopeptidase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of inflammatory response; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis Disease: Myocardial Infarction, Susceptibility To |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P60900 |
| NCBI GenInfo Identifier: | 46397659 |
| NCBI Gene ID: | 5687 |
| NCBI Accession: | P60900.1 |
| UniProt Secondary Accession: | P60900,P34062, Q6IB60, B2R7J9, B4DQR4, B4DXJ9, |
| UniProt Related Accession: | P60900 |
| Molecular Weight: | 246 |
| NCBI Full Name: | Proteasome subunit alpha type-6 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) subunit, alpha type, 6 |
| NCBI Official Symbol: | PSMA6 |
| NCBI Official Synonym Symbols: | IOTA; p27K; PROS27 |
| NCBI Protein Information: | proteasome subunit alpha type-6; PROS-27; macropain iota chain; prosomal P27K protein; proteasome iota chain; macropain subunit iota; 27 kDa prosomal protein; proteasome subunit iota; multicatalytic endopeptidase complex iota chain |
| UniProt Protein Name: | Proteasome subunit alpha type-6 |
| UniProt Synonym Protein Names: | 27 kDa prosomal protein; PROS-27; p27K; Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PSMA6 |
| UniProt Entry Name: | PSA6_HUMAN |
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