Human PSMA1 Recombinant Protein (RPPB2196)

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SKU:
RPPB2196
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
PSMA1
Synonyms:
Proteasome (prosome macropain) subunit alpha type 1
Synonyms:
PROS30
Synonyms:
HC2
Synonyms:
NU
Source:
Escherichia Coli
Uniprot:
P25786
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Description

Product Name:Human PSMA1 Recombinant Protein
Product Code:RPPB2196
Size:20µg
Species:Human
Target:PSMA1
Synonyms:Proteasome (prosome macropain) subunit alpha type 1, PROS30, HC2, NU, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, 30 kDa prosomal protein, protein P30-33K, PROS-30, PSC2, EC 3.4.25.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMA1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMFRNQYD NDVTVWSPQG RIHQIEYAME AVKQGSATVG LKSKTHAVLV ALKRAQSELA AHQKKILHVD NHIGISIAGL TADARLLCNF MRQECLDSRF VFDRPLPVSR LVSLIGSKTQ IPTQRYGRRP YGVGLLIAGY DDMGPHIFQT CPSANYFDCR AMSIGARSQS ARTYLERHMS EFMECNLNEL VKHGLRALRE TLPAEQDLTT KNVSIGIVGK DLEFTIYDDD DVSPFLEGLE ERPQRKAQPA QPADEPAEKA DEPMEH

PSMA1 is a prosomal protein who takes part in a nonlysosomal ATP/ubiquitin-dependent proteolytic pathway. PSMA1 is a multicatalytic proteinase complex that is characterized by its capacity to cleave peptides with Arg, Phe, Tyr, Leu, and Glu next to the leaving group at neutral or slightly basic pH. PSMA1 is vastly expressed in prostate epithelium.

PSMA1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 286 amino acids (1-263) and having a molecular mass of 32.0 kDa.PSMA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMA1: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: nucleoplasm; proteasome complex; polysome; proteasome core complex; centrosome; cytosol; nucleus

Molecular Function:threonine endopeptidase activity; protein binding; RNA binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
UniProt Code:P25786
NCBI GenInfo Identifier:130848
NCBI Gene ID:5682
NCBI Accession:P25786.1
UniProt Secondary Accession:P25786,Q53YE8, Q9BRV9, A8K400,
UniProt Related Accession:P25786
Molecular Weight:263
NCBI Full Name:Proteasome subunit alpha type-1
NCBI Synonym Full Names:proteasome (prosome, macropain) subunit, alpha type, 1
NCBI Official Symbol:PSMA1  
NCBI Official Synonym Symbols:NU; HC2; PROS30; HEL-S-275  
NCBI Protein Information:proteasome subunit alpha type-1; PROS-30; protein P30-33K; proteasome nu chain; macropain subunit C2; macropain subunit nu; proteasome subunit nu; 30 kDa prosomal protein; proteasome component C2; proteasome subunit, alpha-type, 1; epididymis secretory pr
UniProt Protein Name:Proteasome subunit alpha type-1
UniProt Synonym Protein Names:30 kDa prosomal protein; PROS-30; Macropain subunit C2; Multicatalytic endopeptidase complex subunit C2; Proteasome component C2; Proteasome nu chain
Protein Family:Proteasome
UniProt Gene Name:PSMA1  
UniProt Entry Name:PSA1_HUMAN
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