The Human PRSS2 (Protease, Serine 2) ELISA Kit is designed for the accurate detection of PRSS2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.PRSS2 is a serine protease enzyme that plays a crucial role in various physiological processes, including digestion and inflammation regulation. Dysregulation of PRSS2 has been linked to conditions such as pancreatitis and pancreatic cancer, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.
With the Human PRSS2 ELISA Kit, researchers can accurately measure PRSS2 levels in human samples, providing valuable insights into its role in disease pathogenesis and progression. This kit is an essential tool for researchers looking to further understand the functions of PRSS2 and its potential implications in human health.
Product Name:
Human PRSS2 (Protease, Serine, 2) ELISA Kit
SKU:
HUES02250
Target:
Human PRSS2 (Protease, Serine, 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRSS2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRSS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRSS2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRSS2. You can calculate the concentration of Human PRSS2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
96-110
98-112
Average (%)
105
101
103
1:4
Range (%)
87-99
85-97
87-99
Average (%)
94
91
92
1:8
Range (%)
90-101
85-100
89-103
Average (%)
95
91
95
1:16
Range (%)
88-104
85-100
83-96
Average (%)
95
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.57
7.36
19.43
2.5
6.79
21.25
Standard deviation
0.14
0.37
0.78
0.17
0.33
1.13
C V (%)
5.45
5.03
4.01
6.8
4.86
5.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PRSS2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRSS2 in samples. No significant cross-reactivity or interference between Human PRSS2 and analogues was observed.
