Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. In addition, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.
Product Name:
Human Prostasin/PRSS8 Recombinant Protein (RPES4797)
Product Code:
RPES4797
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CAP1,PROSTASIN
Accession:
NP_002764.1
Sequence:
Ala 30-Arg 322
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (R&D Systems, Catalog # ES014). The specific activity is >10 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human Prss8 (NP_002764.1) (Ala 30-Arg 322) was fused with a signal peptide at the N-terminus and a polyhistidine-tag at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
32.8 kDa
AP Mol Mass:
40 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
