The Human PROC (Protein C) ELISA Kit is specifically designed for the precise measurement of Protein C levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for various research purposes.Protein C is a vital protein that plays a crucial role in the regulation of blood coagulation. It functions as a natural anticoagulant, inhibiting blood clot formation and preventing excess clotting.
Dysregulation of Protein C can lead to various thrombotic disorders and increase the risk of thrombosis-related conditions.By measuring Protein C levels, researchers can gain valuable insights into the coagulation process and potentially develop effective treatments for thrombotic disorders. The Human PROC ELISA Kit offers a reliable and efficient solution for studying Protein C and its implications in various diseases and conditions.
Product Name:
Human PROC (Protein C) ELISA Kit
SKU:
HUES02247
Target:
Human PROC (Protein C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PROC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PROC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PROC. You can calculate the concentration of Human PROC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
97-114
93-104
Average (%)
106
105
98
1:4
Range (%)
89-102
78-92
87-101
Average (%)
96
85
94
1:8
Range (%)
94-109
84-96
89-101
Average (%)
100
90
94
1:16
Range (%)
93-105
84-97
89-102
Average (%)
99
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.18
548.62
1879.79
202.95
578.76
1843.26
Standard deviation
12.54
30.67
78.2
10.7
31.2
101.19
C V (%)
6.63
5.59
4.16
5.27
5.39
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PROC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PROC in samples. No significant cross-reactivity or interference between Human PROC and analogues was observed.
