Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation; signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family; and exists as at least homodimers and homotetramers; or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development; as well as histone H3 'Arg-8'; which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally; it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.
Product Name:
Human PRMT5/SKB1 Recombinant Protein (RPES3858)
Product Code:
RPES3858
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
HRMT1L5,IBP72,JBP1,SKB1,SKB1Hs
Accession:
NP_006100.2
Sequence:
Ala 2-Leu 637
Fusion tag:
C-His & N-FLAG
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PRMT5 (NP_006100.2) (Ala 2-Leu 637) was expressed; fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
