The Human PRL (Prolactin) ELISA Kit is specifically designed for the precise measurement of prolactin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for various research applications.Prolactin is a hormone that plays a crucial role in lactation, reproductive health, and immune function. Abnormal levels of prolactin have been associated with conditions such as infertility, menstrual irregularities, and pituitary tumors, making it a valuable biomarker for studying these disorders and developing potential treatments.
With the Human PRL ELISA Kit, researchers can reliably and efficiently analyze prolactin levels in biological samples, providing valuable insights into the role of this hormone in health and disease.
Product Name:
Human PRL (Prolactin) ELISA Kit
SKU:
HUES01416
Target:
Human PRL (Prolactin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRL. You can calculate the concentration of Human PRL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
94-108
85-100
Average (%)
101
102
92
1:4
Range (%)
97-114
83-97
92-105
Average (%)
105
90
98
1:8
Range (%)
97-110
81-92
92-106
Average (%)
103
86
100
1:16
Range (%)
101-117
82-95
95-110
Average (%)
108
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.5
11.9
36.2
5.6
11.7
39.8
Standard deviation
0.3
0.7
1.5
0.3
0.5
1.7
C V (%)
5.45
5.88
4.14
5.36
4.27
4.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PRL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRL in samples. No significant cross-reactivity or interference between Human PRL and analogues was observed.
