Human PRKRA Recombinant Protein (RPPB2635)
- SKU:
- RPPB2635
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PRKRA
- Synonyms:
- Interferon-inducible double-stranded RNA-dependent protein kinase activator A
- Protein Kinase IFN Double Stranded RNA Activator
- PKR-associated protein X
- PKR-associating protein X
- Source:
- Escherichia Coli
- Uniprot:
- O75569
Description
| Product Name: | Human PRKRA Recombinant Protein |
| Product Code: | RPPB2635 |
| Size: | 10µg |
| Species: | Human |
| Target: | PRKRA |
| Synonyms: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A, Protein Kinase IFN Double Stranded RNA Activator, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX, HSD-14, HSD14, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PRKRA solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSQSRHR AEAPPLERED SGTFSLGKMI TAKPGKTPIQ VLHEYGMKTK NIPVYECERS DVQIHVPTFT FRVTVGDITC TGEGTSKKLA KHRAAEAAIN ILKANASICF AVPDPLMPDP SKQPKNQLNP IGSLQELAIH HGWRLPEYTL SQEGGPAHKR EYTTICRLES FMETGKGASK KQAKRNAAEK FLAKFSNISP ENHISLTNVV GHSLGCTWHS LRNSPGEKIN LLKRSLLSIP NTDYIQLLSE IAKEQGFNIT YLDIDELSAN GQYQCLAELS TSPITVCHGS GISCGNAQSD AAHNALQYLK IIAERK |
Protein Kinase IFN Double Stranded RNA Activator, also known as PRKRA, mediates the effects of interferon when a viral infection takes place. PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA) and leads to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Mutations in PRKRA are associated with dystonia.
PRKRA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 336 amino acids (1-313a.a) and having a molecular mass of 36.8kDa.PRKRA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported. |
| UniProt Protein Details: | Protein type:Activator; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 2q31.2 Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol Molecular Function:protein binding; protein homodimerization activity; enzyme activator activity Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; immune response; gene expression; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation Disease: Dystonia 16 |
| NCBI Summary: | This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| UniProt Code: | O75569 |
| NCBI GenInfo Identifier: | 74735517 |
| NCBI Gene ID: | 8575 |
| NCBI Accession: | O75569.1 |
| UniProt Secondary Accession: | O75569,Q53G24, Q6X7T5, Q8NDK4, A8K3I6, |
| UniProt Related Accession: | O75569 |
| Molecular Weight: | 313 |
| NCBI Full Name: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A |
| NCBI Synonym Full Names: | protein kinase, interferon-inducible double stranded RNA dependent activator |
| NCBI Official Symbol: | PRKRA |
| NCBI Official Synonym Symbols: | RAX; PACT; DYT16; HSD14 |
| NCBI Protein Information: | interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A |
| UniProt Protein Name: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A |
| UniProt Synonym Protein Names: | PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator |
| Protein Family: | Interferon-inducible double-stranded RNA-dependent protein kinase activator |
| UniProt Gene Name: | PRKRA |
| UniProt Entry Name: | PRKRA_HUMAN |
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