Human PRKAG1 Recombinant Protein (RPPB2634)
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주문- SKU:
- RPPB2634
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PRKAG1
- Synonyms:
- Protein Kinase AMP-Activated Gamma 1 Non-Catalytic Subunit
- Synonyms:
- 5'-AMP-Activated Protein Kinase Subunit Gamma-1
- Synonyms:
- AMPK Gamma1 Chain
- Synonyms:
- AMPKg
- Source:
- Escherichia Coli
- Uniprot:
- P54619
Description
| Product Name: | Human PRKAG1 Recombinant Protein |
| Product Code: | RPPB2634 |
| Size: | 10µg |
| Species: | Human |
| Target: | PRKAG1 |
| Synonyms: | Protein Kinase AMP-Activated Gamma 1 Non-Catalytic Subunit, 5'-AMP-Activated Protein Kinase Subunit Gamma-1, AMPK Gamma1 Chain, AMPKg. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PRKAG1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMETVISS DSSPAVENEH PQETPESNNS VYTSFMKSHR CYDLIPTSSK LVVFDTSLQV KKAFFALVTN GVRAAPLWDS KKQSFVGMLT ITDFINILHR YYKSALVQIY ELEEHKIETW REVYLQDSFK PLVCISPNAS LFDAVSSLIR NKIHRLPVID PESGNTLYIL THKRILKFLK LFITEFPKPE FMSKSLEELQ IGTYANIAMV RTTTPVYVAL GIFVQHRVSA LPVVDEKGRV VDIYSKFDVI NLAAEKTYNN LDVSVTKALQ HRSHYFEGVL KCYLHETLET IINRLVEAEV HRLVVVDEND VVKGIVSLSD ILQALVLTGG EKKP |
PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer comprised of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is a vital energy-sensing enzyme which supervises cellular energy status. PRKAG1 is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding dissimilar isoforms were found.
PRKAG1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-331) and having a molecular mass of 40.0kDa.PRKAG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes. |
| UniProt Protein Details: | Protein type:Autophagy; Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 12q12-q14 Cellular Component: AMP-activated protein kinase complex; cytosol; membrane; nucleoplasm Molecular Function:ADP binding; AMP binding; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; protein binding; protein kinase activity; protein kinase binding Biological Process: cell cycle arrest; macroautophagy; positive regulation of protein kinase activity; protein amino acid phosphorylation; regulation of glycolysis; signal transduction; spermatogenesis |
| NCBI Summary: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P54619 |
| NCBI GenInfo Identifier: | 1703037 |
| NCBI Gene ID: | 5571 |
| NCBI Accession: | P54619.1 |
| UniProt Secondary Accession: | P54619,Q8N7V9, B4DDT7, |
| UniProt Related Accession: | P54619 |
| Molecular Weight: | 38,533 Da |
| NCBI Full Name: | 5'-AMP-activated protein kinase subunit gamma-1 |
| NCBI Synonym Full Names: | protein kinase AMP-activated non-catalytic subunit gamma 1 |
| NCBI Official Symbol: | PRKAG1 |
| NCBI Official Synonym Symbols: | AMPKG |
| NCBI Protein Information: | 5'-AMP-activated protein kinase subunit gamma-1 |
| UniProt Protein Name: | 5'-AMP-activated protein kinase subunit gamma-1 |
| Protein Family: | 5'-AMP-activated protein kinase |
| UniProt Gene Name: | PRKAG1 |
| UniProt Entry Name: | AAKG1_HUMAN |
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