Human PRKACA Recombinant Protein (RPPB2632)
- SKU:
- RPPB2632
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- PRKACA
- Synonyms:
- cAMP-dependent protein kinase alpha-catalytic subunit
- EC 271111
- PKA C-alpha
- PKACA
- Source:
- Sf9 Insect cells
- Uniprot:
- P17612
Description
| Product Name: | Human PRKACA Recombinant Protein |
| Product Code: | RPPB2632 |
| Size: | 5µg |
| Species: | Human |
| Target: | PRKACA |
| Synonyms: | cAMP-dependent protein kinase alpha-catalytic subunit, EC 2.7.11.11, PKA C-alpha, PKACA, PRKACA, MGC48865, MGC102831. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PRKACA protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMGN AAAAKKGSEQ ESVKEFLAKA KEDFLKKWES PAQNTAHLDQ FERIKTLGTG SFGRVMLVKH KETGNHYAMK ILDKQKVVKL KQIEHTLNEK RILQAVNFPF LVKLEFSFKD NSNLYMVMEY VPGGEMFSHL RRIGRFSEPH ARFYAAQIVL TFEYLHSLDL IYRDLKPENL LIDQQGYIQV TDFGFAKRVK GRTWTLCGTP EYLAPEIILS KGYNKAVDWW ALGVLIYEMA AGYPPFFADQ PIQIYEKIVS GKVRFPSHFS SDLKDLLRNL LQVDLTKRFG NLKNGVNDIK NHKWFATTDW IAIYQRKVEA PFIPKFKGPG DTSNFDDYEE EEIRVSINEK CGKEFSEF |
cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1 (PRKACA) belongs to the Ser/Thr protein kinase family. PRKACA is responsible for phosphorylating other proteins and substrates, changing their activity. The PRKACA protein is a member of the AGC kinase family, and contributes to the regulation of cellular processes which include glucose metabolism, cell division, and contextual memory.
PRKACA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 578 amino acids (1-351 a.a.) and having a molecular mass of 67kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions). PRKACA is expressed with a 227 amino acid GST Tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; AGC group; PKA family Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: centrosome; membrane; mitochondrion; plasma membrane; cytosol; nucleus; neuromuscular junction; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex Molecular Function:protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding Biological Process: nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; water transport; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; regulation of synaptic transmission; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytosolic calcium ion homeostasis; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; neural tube closure; carbohydrate metabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; regulation of insulin secretion |
| NCBI Summary: | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P17612 |
| NCBI GenInfo Identifier: | 125205 |
| NCBI Gene ID: | 5566 |
| NCBI Accession: | P17612.2 |
| UniProt Secondary Accession: | P17612,Q32P54, Q9H2Y0, Q9NRB4, Q9NRH9, |
| UniProt Related Accession: | P17612 |
| Molecular Weight: | 39,953 Da |
| NCBI Full Name: | cAMP-dependent protein kinase catalytic subunit alpha |
| NCBI Synonym Full Names: | protein kinase, cAMP-dependent, catalytic, alpha |
| NCBI Official Symbol: | PRKACA |
| NCBI Official Synonym Symbols: | PKACA |
| NCBI Protein Information: | cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; protein kinase A catalytic subunit; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1 |
| UniProt Protein Name: | cAMP-dependent protein kinase catalytic subunit alpha |
| Protein Family: | cAMP-dependent protein kinase |
| UniProt Gene Name: | PRKACA |
| UniProt Entry Name: | KAPCA_HUMAN |
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