The Human PR3 (Proteinase 3) ELISA Kit is specifically designed for the precise detection of PR3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are suitable for a variety of research purposes.PR3, also known as Proteinase 3, is a critical enzyme involved in immune responses and inflammatory processes. Its dysregulation has been linked to various autoimmune diseases, such as vasculitis and rheumatoid arthritis, highlighting its importance as a biomarker for studying these conditions and exploring potential treatment options.
Overall, the Human PR3 ELISA Kit provides a valuable tool for researchers looking to investigate the role of PR3 in human health and disease, offering a reliable method for quantifying PR3 levels in biological samples.
Product Name:
Human PR3 (Proteinase 3) ELISA Kit
SKU:
HUES02909
Target:
Human PR3 (Proteinase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PR3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PR3. You can calculate the concentration of Human PR3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
99-113
93-105
Average (%)
93
106
99
1:4
Range (%)
86-101
85-98
82-96
Average (%)
93
91
88
1:8
Range (%)
90-106
86-99
80-95
Average (%)
97
92
87
1:16
Range (%)
93-106
87-100
87-97
Average (%)
99
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.34
4.12
0.51
1.4
4.05
Standard deviation
0.02
0.06
0.18
0.03
0.08
0.15
C V (%)
4.26
4.48
4.37
5.88
5.71
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PR3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PR3 in samples. No significant cross-reactivity or interference between Human PR3 and analogues was observed.
