Human PPP1R14A Recombinant Protein (RPPB2165)
- SKU:
- RPPB2165
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PPP1R14A
- Synonyms:
- Protein phosphatase 1 regulatory subunit 14A
- 17 kDa PKC-potentiated inhibitory protein of PP1
- CPI17
- CPI-17
- Source:
- Escherichia Coli
- Uniprot:
- Q96A00
Description
| Product Name: | Human PPP1R14A Recombinant Protein |
| Product Code: | RPPB2165 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPP1R14A |
| Synonyms: | Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, CPI17, CPI-17, PPP1INL, PPP1R14A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PPP1R14A protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8, 0.2mM EDTA, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP |
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. Inhibition of PPP1R14A results to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.Human pregnancy is characterized by the increases of PKN1 expression and CPI-17 phosphorylation in the myometrium. PPP1R14A is mapped to chromosome 19q13.13-q13.2.PPP1R14A binds directly to protein kinase C and casein kinase I. PPP1R14A siRNA decreased the level of merlin phosphorylation and consequently Ras and ERK activity in human tumor cell lines. PKC/CPI-17 mediated pathway in histamine, triggeres cytoskeletal rearrangements causing lung microvascular barrier compromise.
Recombinant PPP1R14A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids and having a molecular mass of 18kDa. PPP1R14A is fused to a 20 aa His Tag and is purified by conventional chromatography techniques.
| UniProt Protein Function: | PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A. |
| UniProt Protein Details: | Protein type:Inhibitor; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: cytosol Molecular Function:protein phosphatase inhibitor activity Biological Process: negative regulation of catalytic activity; regulation of phosphorylation; small GTPase mediated signal transduction |
| NCBI Summary: | The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q96A00 |
| NCBI GenInfo Identifier: | 55583974 |
| NCBI Gene ID: | 94274 |
| NCBI Accession: | Q96A00.1 |
| UniProt Secondary Accession: | Q96A00,Q7Z4X7, Q96S54, |
| UniProt Related Accession: | Q96A00 |
| Molecular Weight: | 13,480 Da |
| NCBI Full Name: | Protein phosphatase 1 regulatory subunit 14A |
| NCBI Synonym Full Names: | protein phosphatase 1 regulatory inhibitor subunit 14A |
| NCBI Official Symbol: | PPP1R14A |
| NCBI Official Synonym Symbols: | CPI17; CPI-17; PPP1INL |
| NCBI Protein Information: | protein phosphatase 1 regulatory subunit 14A |
| UniProt Protein Name: | Protein phosphatase 1 regulatory subunit 14A |
| UniProt Synonym Protein Names: | 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17 |
| UniProt Gene Name: | PPP1R14A |
| UniProt Entry Name: | PP14A_HUMAN |
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