The Human PPARgammaC1-alpha (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha) ELISA Kit offered by Assay Genie is specifically designed for the precise detection of PPARgammaC1-alpha levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PPARgammaC1-alpha is a critical transcriptional coactivator involved in the regulation of various metabolic processes, including energy metabolism and mitochondrial function.
Dysregulation of PPARgammaC1-alpha has been linked to metabolic disorders, such as obesity and diabetes, as well as neurodegenerative diseases and cancer.By utilizing Assay Genie's Human PPARgammaC1-alpha ELISA Kit, researchers can gain valuable insights into the role of PPARgammaC1-alpha in health and disease, paving the way for advancements in therapeutic interventions and personalized medicine.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPARγC1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPARγC1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPARγC1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PPARγC1α. You can calculate the concentration of Human PPARγC1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
91-104
95-109
Average (%)
104
97
101
1:4
Range (%)
88-105
79-93
83-93
Average (%)
96
85
88
1:8
Range (%)
89-102
82-94
86-100
Average (%)
95
89
93
1:16
Range (%)
86-102
80-90
89-103
Average (%)
93
85
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
96
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.32
4.51
0.47
1.43
4.38
Standard deviation
0.03
0.06
0.15
0.03
0.08
0.19
C V (%)
5.88
4.55
3.33
6.38
5.59
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PPARγC1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PPARγC1α in samples. No significant cross-reactivity or interference between Human PPARγC1α and analogues was observed.
