Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
The Human PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) ELISA Kit is specifically designed for the accurate and reliable detection of PPAR Gamma levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for a variety of research applications.PPAR Gamma is a key transcription factor involved in the regulation of numerous biological processes, including lipid metabolism, insulin sensitivity, and inflammatory responses.
Dysregulation of PPAR Gamma has been linked to various diseases such as obesity, diabetes, and cardiovascular disorders, making it a vital target for drug development and therapeutic interventions.By utilizing the Human PPAR Gamma ELISA Kit, researchers can gain valuable insights into the role of PPAR Gamma in various physiological and pathological conditions, ultimately advancing our understanding of disease mechanisms and potential treatment options. Elevate your research with this innovative and reliable ELISA kit from Assay Genie.
Product Name:
Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
SKU:
HUES02406
Target:
Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PPAR-γ. You can calculate the concentration of Human PPAR-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
93-110
99-114
Average (%)
94
101
105
1:4
Range (%)
86-102
83-96
87-103
Average (%)
93
89
94
1:8
Range (%)
92-105
85-98
87-99
Average (%)
97
90
94
1:16
Range (%)
88-99
82-97
82-93
Average (%)
94
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.12
4.88
0.46
1.15
4.44
Standard deviation
0.03
0.07
0.17
0.03
0.07
0.24
C V (%)
6.38
6.25
3.48
6.52
6.09
5.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PPAR-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PPAR-γ in samples. No significant cross-reactivity or interference between Human PPAR-γ and analogues was observed.
