The Human PP (Pepsin) ELISA Kit is specifically designed for the quantitative measurement of Pepsin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Pepsin is a key enzyme involved in the digestion process, breaking down proteins in the stomach. Abnormal levels of Pepsin have been associated with conditions such as gastroesophageal reflux disease (GERD) and peptic ulcers, making it an important biomarker for studying and diagnosing these conditions.
By accurately measuring Pepsin levels, researchers and healthcare professionals can gain valuable insights into gastrointestinal health and potentially develop new diagnostic and therapeutic strategies for Pepsin-related disorders.
Product Name:
Human PP (Pepsin) ELISA Kit
SKU:
HUES02968
Target:
Human PP (Pepsin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PP. You can calculate the concentration of Human PP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
83-96
94-105
Average (%)
99
90
100
1:4
Range (%)
90-100
82-92
87-101
Average (%)
95
87
93
1:8
Range (%)
94-108
82-95
85-99
Average (%)
100
88
92
1:16
Range (%)
93-106
82-95
85-96
Average (%)
99
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
203.39
424.25
1389.47
218.85
397.39
1334.35
Standard deviation
12.37
25.46
50.02
11.91
18.44
72.86
C V (%)
6.08
6.0
3.6
5.44
4.64
5.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PP in samples. No significant cross-reactivity or interference between Human PP and analogues was observed.
