Human POLR3F Recombinant Protein (RPPB2139)
- SKU:
- RPPB2139
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- POLR3F
- Synonyms:
- DNA-directed RNA polymerase III subunit RPC6
- RNA polymerase III subunit C6
- DNA-directed RNA polymerase III subunit F
- RNA polymerase III 39 kDa subunit
- Source:
- Escherichia Coli
- Uniprot:
- Q9H1D9
Description
| Product Name: | Human POLR3F Recombinant Protein |
| Product Code: | RPPB2139 |
| Size: | 20µg |
| Species: | Human |
| Target: | POLR3F |
| Synonyms: | DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III subunit C6, DNA-directed RNA polymerase III subunit F, RNA polymerase III 39 kDa subunit, RPC39, POLR3F, RPC6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The POLR3F solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF |
Polymerase III Polypeptide F (POLR3F) is a member of the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. POLR3F is one of many subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. POLR3F binds both TFIIIB90 and TBP, 2 subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB).
POLR3F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316) and having a molecular mass of 38.1kDa.POLR3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex Chromosomal Location of Human Ortholog: 20p11.23 Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm Molecular Function:protein binding Biological Process: positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production |
| NCBI Summary: | The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| UniProt Code: | Q9H1D9 |
| NCBI GenInfo Identifier: | 20139728 |
| NCBI Gene ID: | 10621 |
| NCBI Accession: | Q9H1D9.1 |
| UniProt Secondary Accession: | Q9H1D9,O15319, A8K4C7, |
| UniProt Related Accession: | Q9H1D9 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | DNA-directed RNA polymerase III subunit RPC6 |
| NCBI Synonym Full Names: | RNA polymerase III subunit F |
| NCBI Official Symbol: | POLR3F |
| NCBI Official Synonym Symbols: | RPC6; RPC39 |
| NCBI Protein Information: | DNA-directed RNA polymerase III subunit RPC6 |
| UniProt Protein Name: | DNA-directed RNA polymerase III subunit RPC6 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase III subunit F; RNA polymerase III 39 kDa subunit; RPC39 |
| UniProt Gene Name: | POLR3F |
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