Human POLR2C Recombinant Protein (RPPB4272)
- SKU:
- RPPB4272
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- POLR2C
- Synonyms:
- hRPB33
- hsRPB3
- RPB3
- RPB31
- Source:
- Escherichia Coli
- Uniprot:
- P19387
Description
| Product Name: | Human POLR2C Recombinant Protein |
| Product Code: | RPPB4272 |
| Size: | 20µg |
| Species: | Human |
| Target: | POLR2C |
| Synonyms: | hRPB33, hsRPB3, RPB3, RPB31, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, RPB33, DNA-directed RNA polymerase II subunit C, POLR2C. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | POLR2C protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol 1mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMPYANQP TVRITELTDE NVKFIIENTD LAVANSIRRV FIAEVPIIAI DWVQIDANSS VLHDEFIAHR LGLIPLISDD IVDKLQYSRD CTCEEFCPEC SVEFTLDVRC NEDQTRHVTS RDLISNSPRV IPVTSRNRDN DPNDYVEQDD ILIVKLRKGQ ELRLRAYAKK GFGKEHAKWN PTAGVAFEYD PDNALRHTVY PKPEEWPKSE YSELDEDESQ APYDPNGKPE RFYYNVESCG SLRPETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN |
Polymerase (RNA) II (DNA directed) Polypeptide C (POLR2C) is the 3rd largest subunit of RNA polymerase II. The product of POLR2C consists of a cysteine rich area and exists as a heterodimer with an additional polymerase subunit, POLR2J. Those 2 subunits shape a core subassembly unit of the polymerase.
POLR2C Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275a.a) and having a molecular mass of 33.8kDa. POLR2C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLR2C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:DNA-binding; Nucleotide Metabolism - purine; Transcription initiation complex; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 16q13-q21 Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:protein dimerization activity; DNA binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; nucleotide-excision repair; somatic stem cell maintenance; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair |
| NCBI Summary: | This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19387 |
| NCBI GenInfo Identifier: | 3915850 |
| NCBI Gene ID: | 5432 |
| NCBI Accession: | P19387.2 |
| UniProt Related Accession: | P19387 |
| Molecular Weight: | |
| NCBI Full Name: | DNA-directed RNA polymerase II subunit RPB3 |
| NCBI Synonym Full Names: | RNA polymerase II subunit C |
| NCBI Official Symbol: | POLR2C |
| NCBI Official Synonym Symbols: | RPB3; RPB31; hRPB33; hsRPB3 |
| NCBI Protein Information: | DNA-directed RNA polymerase II subunit RPB3 |
| UniProt Protein Name: | DNA-directed RNA polymerase II subunit RPB3 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II 33 kDa polypeptide; RPB33; DNA-directed RNA polymerase II subunit C; RPB31 |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR2C |
| UniProt Entry Name: | RPB3_HUMAN |
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