Human POLL Recombinant Protein (RPPB2135)
- SKU:
- RPPB2135
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- POLL
- Synonyms:
- Polymerase (DNA directed) lambda
- Pol beta2
- DNA polymerase beta-2
- DNA polymerase kappa
- Source:
- Escherichia Coli
- Uniprot:
- Q9UGP5
Description
| Product Name: | Human POLL Recombinant Protein |
| Product Code: | RPPB2135 |
| Size: | 10µg |
| Species: | Human |
| Target: | POLL |
| Synonyms: | Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW |
DNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5’-deoxyribose-5-phosphate lyase activity which is synchronized by PCNA and replication protein A.
POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family. |
| UniProt Protein Details: | Protein type:EC 2.7.7.7; DNA repair, damage; Transferase; Lyase Chromosomal Location of Human Ortholog: 10q23 Cellular Component: nucleoplasm Molecular Function:DNA-directed DNA polymerase activity Biological Process: double-strand break repair via nonhomologous end joining; nucleotide-excision repair |
| NCBI Summary: | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q9UGP5 |
| NCBI GenInfo Identifier: | 17367126 |
| NCBI Gene ID: | 27343 |
| NCBI Accession: | Q9UGP5.1 |
| UniProt Secondary Accession: | Q9UGP5,Q5JQP5, Q6NUM2, Q9BTN8, Q9HA10, Q9HB35, D3DR76 |
| UniProt Related Accession: | Q9UGP5 |
| Molecular Weight: | 33,839 Da |
| NCBI Full Name: | DNA polymerase lambda |
| NCBI Synonym Full Names: | polymerase (DNA) lambda |
| NCBI Official Symbol: | POLL |
| NCBI Official Synonym Symbols: | BETAN; POLKAPPA |
| NCBI Protein Information: | DNA polymerase lambda |
| UniProt Protein Name: | DNA polymerase lambda |
| UniProt Synonym Protein Names: | DNA polymerase beta-2; Pol beta2; DNA polymerase kappa |
| Protein Family: | Pollen allergen |
| UniProt Gene Name: | POLL |
| UniProt Entry Name: | DPOLL_HUMAN |
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