Human POLL Recombinant Protein (RPPB2135)

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SKU:
RPPB2135
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
POLL
Synonyms:
Polymerase (DNA directed) lambda
Synonyms:
Pol beta2
Synonyms:
DNA polymerase beta-2
Synonyms:
DNA polymerase kappa
Source:
Escherichia Coli
Uniprot:
Q9UGP5
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Description

Product Name:Human POLL Recombinant Protein
Product Code:RPPB2135
Size:10µg
Species:Human
Target:POLL
Synonyms:Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW

DNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5’-deoxyribose-5-phosphate lyase activity which is synchronized by PCNA and replication protein A.

POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.
UniProt Protein Details:

Protein type:EC 2.7.7.7; DNA repair, damage; Transferase; Lyase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm

Molecular Function:DNA-directed DNA polymerase activity

Biological Process: double-strand break repair via nonhomologous end joining; nucleotide-excision repair

NCBI Summary:This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
UniProt Code:Q9UGP5
NCBI GenInfo Identifier:17367126
NCBI Gene ID:27343
NCBI Accession:Q9UGP5.1
UniProt Secondary Accession:Q9UGP5,Q5JQP5, Q6NUM2, Q9BTN8, Q9HA10, Q9HB35, D3DR76
UniProt Related Accession:Q9UGP5
Molecular Weight:33,839 Da
NCBI Full Name:DNA polymerase lambda
NCBI Synonym Full Names:polymerase (DNA) lambda
NCBI Official Symbol:POLL  
NCBI Official Synonym Symbols:BETAN; POLKAPPA  
NCBI Protein Information:DNA polymerase lambda
UniProt Protein Name:DNA polymerase lambda
UniProt Synonym Protein Names:DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Protein Family:Pollen allergen
UniProt Gene Name:POLL  
UniProt Entry Name:DPOLL_HUMAN
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