Human PMVK Recombinant Protein (RPPB2623)
- SKU:
- RPPB2623
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PMVK
- Synonyms:
- Phosphomevalonate kinase
- PMKase
- hPMK
- PMVK
- Source:
- Escherichia Coli
- Uniprot:
- Q15126
Description
| Product Name: | Human PMVK Recombinant Protein |
| Product Code: | RPPB2623 |
| Size: | 20µg |
| Species: | Human |
| Target: | PMVK |
| Synonyms: | Phosphomevalonate kinase, PMKase, hPMK, PMVK, PMKI, PMK, PMKA, PMKASE, HUMPMKI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PMVK solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL |
Phosphomevalonate kinase (PMVK) is a cytosolic enzyme. PMVK is expressed highly in the heart, liver, skeletal muscle, kidney and pancreas and slightly lower in the brain, placenta, and lung. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway.
PMVK Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 212 amino acids (1-192 a.a.) and having a molecular mass of 24.1kDa. The PMVK is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PMVK: Induced by sterol. Monomer. |
| UniProt Protein Details: | Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.7.4.2; Kinase, other Chromosomal Location of Human Ortholog: 1q22 Cellular Component: membrane; peroxisome; cytosol Molecular Function:phosphomevalonate kinase activity; ATP binding Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process |
| NCBI Summary: | This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q15126 |
| NCBI GenInfo Identifier: | 3024422 |
| NCBI Gene ID: | 10654 |
| NCBI Accession: | Q15126.3 |
| UniProt Secondary Accession: | Q15126,Q5TZW9, |
| UniProt Related Accession: | Q15126 |
| Molecular Weight: | 21,995 Da |
| NCBI Full Name: | Phosphomevalonate kinase |
| NCBI Synonym Full Names: | phosphomevalonate kinase |
| NCBI Official Symbol: | PMVK |
| NCBI Official Synonym Symbols: | PMK; PMKA; PMKASE; HUMPMKI |
| NCBI Protein Information: | phosphomevalonate kinase; hPMK |
| UniProt Protein Name: | Phosphomevalonate kinase |
| UniProt Gene Name: | PMVK |
| UniProt Entry Name: | PMVK_HUMAN |
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