Human PLTP (Phospholipid Transfer Protein) ELISA Kit
The Human PLTP (Phospholipid Transfer Protein) ELISA Kit is specifically designed for the accurate measurement of PLTP levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for various research applications.PLTP is a key protein that plays a critical role in lipid metabolism and lipid transport. It is involved in the transfer of phospholipids between lipoproteins, contributing to the maintenance of lipid homeostasis in the body.
Dysregulation of PLTP has been linked to various metabolic disorders, including atherosclerosis and metabolic syndrome.By accurately measuring PLTP levels, researchers can gain valuable insights into lipid metabolism, cardiovascular health, and metabolic disorders. The Human PLTP ELISA Kit provides a powerful tool for studying the role of PLTP in health and disease, ultimately leading to the development of potential diagnostic and therapeutic strategies.
Product Name:
Human PLTP (Phospholipid Transfer Protein) ELISA Kit
SKU:
HUES02705
Target:
Human PLTP (Phospholipid Transfer Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLTP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLTP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLTP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLTP. You can calculate the concentration of Human PLTP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
90-104
86-97
Average (%)
105
95
91
1:4
Range (%)
91-105
86-98
87-102
Average (%)
99
93
94
1:8
Range (%)
90-107
87-98
82-93
Average (%)
97
92
88
1:16
Range (%)
91-107
79-93
86-100
Average (%)
98
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.35
9.55
0.94
2.43
9.35
Standard deviation
0.07
0.13
0.49
0.06
0.12
0.34
C V (%)
6.93
5.53
5.13
6.38
4.94
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PLTP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLTP in samples. No significant cross-reactivity or interference between Human PLTP and analogues was observed.
