The Human PLG (Plasminogen) ELISA Kit is specifically developed to accurately measure levels of plasminogen in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring the reliability and consistency of results for various research purposes.Plasminogen is a key protein involved in the breakdown of blood clots and fibrin, crucial for maintaining hemostasis and proper blood flow in the body. Dysregulation of plasminogen levels has been linked to various disorders such as thrombosis, atherosclerosis, and liver diseases, highlighting its importance as a valuable biomarker in understanding these conditions and potential therapeutic interventions.
With the Human PLG ELISA Kit, researchers can accurately quantify plasminogen levels in human samples, facilitating in-depth investigations into the role of this protein in health and disease. This kit is an essential tool for studying plasminogen-related pathways and developing targeted treatments for associated disorders.
Product Name:
Human Plg (Plasminogen) ELISA Kit
SKU:
HUES03011
Target:
Human Plg (Plasminogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Plg. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Plg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Plg, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Plg. You can calculate the concentration of Human Plg in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
93-108
99-115
Average (%)
101
100
105
1:4
Range (%)
87-99
87-99
89-100
Average (%)
93
92
95
1:8
Range (%)
87-101
85-99
89-102
Average (%)
93
92
94
1:16
Range (%)
88-103
82-95
87-99
Average (%)
94
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.34
5.79
24.23
2.4
5.32
24.04
Standard deviation
0.16
0.27
1.08
0.14
0.29
1.23
C V (%)
6.84
4.66
4.46
5.83
5.45
5.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Plg concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Plg in samples. No significant cross-reactivity or interference between Human Plg and analogues was observed.
