Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit
The Human PLAP (ALPP) ELISA Kit is a specialized assay designed for the precise measurement of placental alkaline phosphatase levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications. Placental alkaline phosphatase is a key enzyme that plays a critical role in cellular activities and is implicated in various diseases and disorders.
Studying placental alkaline phosphatase levels can provide valuable insights into conditions such as pregnancy complications, bone disorders, and certain cancers. With its reliable performance and robust design, the Human PLAP (ALPP) ELISA Kit is a valuable tool for researchers seeking to understand the implications of placental alkaline phosphatase in health and disease.
Product Name:
Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit
SKU:
HUES02914
Target:
Human PLAP/ALPP (Placental Alkaline Phosphatase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
87-99
86-100
Average (%)
106
92
93
1:4
Range (%)
92-105
81-94
84-94
Average (%)
99
87
89
1:8
Range (%)
86-100
85-96
84-98
Average (%)
92
91
90
1:16
Range (%)
87-98
86-101
84-98
Average (%)
93
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.03
3.53
0.56
1.04
3.68
Standard deviation
0.03
0.06
0.12
0.04
0.04
0.13
C V (%)
5.77
5.83
3.4
7.14
3.85
3.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PLAP/ALPP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLAP/ALPP in samples. No significant cross-reactivity or interference between Human PLAP/ALPP and analogues was observed.
