Human PLA2R1 (Phospholipase A2 Receptor 1) ELISA Kit
The Human PLA2R1 (Phospholipase A2 Receptor 1) ELISA Kit is specifically designed to accurately measure levels of PLA2R1 in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for various research applications.PLA2R1 is a key receptor involved in phospholipase A2 activity, playing a critical role in inflammatory responses, lipid metabolism, and immune regulation. Dysregulation of PLA2R1 has been linked to various diseases, including autoimmune disorders and cardiovascular conditions, making it an important biomarker for understanding disease pathology and potential therapeutic targets.
This ELISA kit offers researchers a precise and efficient method for quantifying PLA2R1 levels, allowing for better insights into its biological functions and implications in disease progression. Whether studying autoimmune diseases, cardiovascular disorders, or lipid metabolism, the Human PLA2R1 ELISA Kit provides a valuable tool for advancing research in these areas.
Product Name:
Human PLA2R1 (Phospholipase A2 Receptor 1) ELISA Kit
SKU:
HUES02161
Target:
Human PLA2R1 (Phospholipase A2 Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLA2R1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLA2R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA2R1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLA2R1. You can calculate the concentration of Human PLA2R1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
90-103
97-113
Average (%)
104
97
105
1:4
Range (%)
91-103
79-90
88-102
Average (%)
96
85
94
1:8
Range (%)
93-107
85-99
83-96
Average (%)
99
90
90
1:16
Range (%)
89-102
80-96
89-100
Average (%)
94
87
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.28
4.98
0.43
1.16
4.94
Standard deviation
0.02
0.06
0.22
0.03
0.06
0.19
C V (%)
4.26
4.69
4.42
6.98
5.17
3.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PLA2R1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed.
