Human PLA2G2A Recombinant Protein (RPPB2121)
- SKU:
- RPPB2121
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PLA2G2A
- Synonyms:
- MOM1
- PLA2
- PLA2B
- PLA2L
- Source:
- Escherichia Coli
- Uniprot:
- P14555
Description
| Product Name: | Human PLA2G2A Recombinant Protein |
| Product Code: | RPPB2121 |
| Size: | 10µg |
| Species: | Human |
| Target: | PLA2G2A |
| Synonyms: | MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, Phospholipase A2 membrane associated, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IIA phospholipase A2, GIIC sPLA2, Non-pancreatic secretory phospholipase A2, NPS-PLA2, sPLA2-IIA, PLA2G2A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. |
| Solubility: | It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, an intensive dilution by relevant buffer to a concentration of 10?g/ml is recomended. In higher concentrations the solubility of the PLA2G2A antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC |
Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.sPLA2-IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of sPLA2-IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.
Secreted Phospholipase A2-IIA Human Recombinant is manufactured with N-terminal fusion of HisTag. PLA2G2A His-Tagged Fusion Protein is 15.8 kDa containing 124 amino acid residues of the human secreted phospholipase A2-IIA and 16 additional amino acid residues – HisTag.
| UniProt Protein Function: | PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Vesicle; Endoplasmic reticulum; Phospholipase; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Mitochondrial; Secreted; Lipid Metabolism - linoleic acid Chromosomal Location of Human Ortholog: 1p35 Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule Molecular Function:phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding Biological Process: defense response to Gram-positive bacterium; phosphatidic acid metabolic process; somatic stem cell maintenance; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; positive regulation of inflammatory response; negative regulation of epithelial cell proliferation Disease: Colorectal Cancer |
| NCBI Summary: | The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009] |
| UniProt Code: | P14555 |
| NCBI GenInfo Identifier: | 129483 |
| NCBI Gene ID: | 5320 |
| NCBI Accession: | P14555.2 |
| UniProt Secondary Accession: | P14555,Q6DN24, Q6IBD9, Q9UCD2, A8K5I7, |
| UniProt Related Accession: | P14555 |
| Molecular Weight: | 144 |
| NCBI Full Name: | Phospholipase A2, membrane associated |
| NCBI Synonym Full Names: | phospholipase A2, group IIA (platelets, synovial fluid) |
| NCBI Official Symbol: | PLA2G2A |
| NCBI Official Synonym Symbols: | MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2 |
| NCBI Protein Information: | phospholipase A2, membrane associated; NPS-PLA2; GIIC sPLA2; group IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; non-pancreatic secretory phospholipase A2 |
| UniProt Protein Name: | Phospholipase A2, membrane associated |
| UniProt Synonym Protein Names: | GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A |
| Protein Family: | Phospholipase |
| UniProt Gene Name: | PLA2G2A |
| UniProt Entry Name: | PA2GA_HUMAN |
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