The Human PL (Pancreatic Lipase) ELISA Kit is specifically designed for the precise measurement of pancreatic lipase levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an indispensable tool for a variety of research endeavors.Pancreatic lipase is a key enzyme involved in the digestion and absorption of dietary fats, playing a crucial role in maintaining proper lipid metabolism.
Dysregulation of pancreatic lipase levels has been linked to various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and identifying potential therapeutic interventions.With its user-friendly format and trustworthy performance, the Human PL ELISA Kit is a valuable asset for researchers seeking to explore the role of pancreatic lipase in health and disease. Visit the provided URL to learn more and acquire this essential research tool.
Product Name:
Human PL (Pancreatic Lipase) ELISA Kit
SKU:
HUES03131
Target:
Human PL (Pancreatic Lipase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PL. You can calculate the concentration of Human PL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
92-104
98-111
Average (%)
95
98
103
1:4
Range (%)
91-104
82-95
87-101
Average (%)
96
89
92
1:8
Range (%)
90-105
82-97
89-100
Average (%)
97
88
94
1:16
Range (%)
89-104
82-98
85-96
Average (%)
96
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.11
56.36
235.01
28.09
54.59
245.83
Standard deviation
1.47
2.98
11.82
1.95
2.63
9.66
C V (%)
5.63
5.29
5.03
6.94
4.82
3.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PL in samples. No significant cross-reactivity or interference between Human PL and analogues was observed.
