Human PKLR Recombinant Protein (RPPB2620)

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SKU:
RPPB2620
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
PKLR
Synonyms:
PK1
Synonyms:
PKL
Synonyms:
RPK
Synonyms:
pyruvate kinase isozyme R/L
Source:
Escherichia Coli
Uniprot:
P30613
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Description

Product Name:Human PKLR Recombinant Protein
Product Code:RPPB2620
Size:10µg
Species:Human
Target:PKLR
Synonyms:PK1, PKL, RPK, pyruvate kinase isozyme R/L, Red cell/liver pyruvate kinase, PKRL
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The PKLR protein solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT 0.2M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
Biological Activity:Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.

PKLR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 549 amino acids (47-574a.a.) and having a molecular wieght of 59.2kDa. The PKLR is fused to 21a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; EC 2.7.1.40; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytosol

Molecular Function:pyruvate kinase activity

Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells

NCBI Summary:The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P30613
NCBI GenInfo Identifier:8247933
NCBI Gene ID:5313
NCBI Accession:P30613.2
UniProt Secondary Accession:P30613,O75758, P11973,
UniProt Related Accession:P30613
Molecular Weight:58,494 Da
NCBI Full Name:Pyruvate kinase PKLR
NCBI Synonym Full Names:pyruvate kinase, liver and RBC
NCBI Official Symbol:PKLR  
NCBI Official Synonym Symbols:PK1; PKL; PKR; RPK; PKRL  
NCBI Protein Information:pyruvate kinase PKLR
UniProt Protein Name:Pyruvate kinase PKLR
UniProt Synonym Protein Names:Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Protein Family:Pyruvate kinase
UniProt Gene Name:PKLR  
UniProt Entry Name:KPYR_HUMAN
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