Human PIIINP (N-Terminal Procollagen 3 Propeptide) ELISA Kit
The Human PIIINP (N-terminal procollagen 3 propeptide) ELISA Kit is specifically designed for the quantitative measurement of PIIINP levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.PIIINP is a key biomarker for collagen synthesis and remodeling, providing valuable insights into conditions such as fibrosis, liver disease, and bone disorders.
By detecting and measuring PIIINP levels, researchers can gain a deeper understanding of these conditions and potentially identify new therapeutic targets.Whether studying disease mechanisms, monitoring treatment efficacy, or conducting basic research, the Human PIIINP ELISA Kit offers a powerful tool for investigating collagen metabolism and related pathologies. Trust in its precision and performance to advance your research goals.
Product Name:
Human PIIINP (N-Terminal Procollagen 3 Propeptide) ELISA Kit
SKU:
HUES01450
Target:
Human PIIINP (N-Terminal Procollagen 3 Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. You can calculate the concentration of Human PⅢNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
86-98
90-103
Average (%)
101
93
97
1:4
Range (%)
96-106
87-102
95-110
Average (%)
101
93
101
1:8
Range (%)
99-113
83-96
92-108
Average (%)
105
89
99
1:16
Range (%)
94-106
88-101
98-111
Average (%)
100
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
96-107
102
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
75.4
208.1
518.3
69.8
205.9
509.5
Standard deviation
4.8
11.4
18.7
3.9
9.9
18.3
C V (%)
6.37
5.48
3.61
5.59
4.81
3.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PⅢNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PⅢNP in samples. No significant cross-reactivity or interference between Human PⅢNP and analogues was observed.
