Human PIICP (Procollagen II C-Terminal ProPeptide) ELISA Kit
The Human PIICP (Procollagen II C-terminal Propeptide) ELISA Kit is a powerful tool for the precise measurement of PIICP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PIICP is a vital component in collagen synthesis and is specifically involved in the production of type II collagen, which is essential for maintaining healthy cartilage and connective tissues.
Dysregulation of PIICP levels has been linked to conditions such as osteoarthritis, rheumatoid arthritis, and other musculoskeletal disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human PIICP ELISA Kit provides researchers with a reliable method for investigating PIICP levels in human samples, offering insights into the pathophysiology of various diseases and opening avenues for novel treatment strategies.
Product Name:
Human PIICP (Procollagen II C-Terminal ProPeptide) ELISA Kit
SKU:
HUES02102
Target:
Human PIICP (Procollagen II C-Terminal ProPeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅡCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅡCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅡCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅡCP. You can calculate the concentration of Human PⅡCP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
88-101
99-113
Average (%)
91
95
105
1:4
Range (%)
91-105
85-99
87-100
Average (%)
99
90
94
1:8
Range (%)
86-102
85-100
87-102
Average (%)
93
92
93
1:16
Range (%)
91-102
80-95
87-99
Average (%)
97
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.7
282.01
789.95
109.19
293.79
825.95
Standard deviation
6.52
14.41
34.68
7.28
16.75
27.26
C V (%)
5.89
5.11
4.39
6.67
5.7
3.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PⅡCP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PⅡCP in samples. No significant cross-reactivity or interference between Human PⅡCP and analogues was observed.
