Human PIICP (Procollagen II C-Terminal ProPeptide) CLIA Kit
The Human PIICP (Procollagen II C-terminal propeptide) CLIA Kit is specifically designed for the quantitative detection of PIICP levels in human serum and plasma samples. This kit offers high sensitivity and accuracy, providing reliable and reproducible results for research purposes.PIICP is an important marker for collagen synthesis and cartilage formation, making it a valuable biomarker for studying conditions related to cartilage degradation, such as osteoarthritis. By accurately measuring PIICP levels, researchers can gain valuable insights into the mechanisms underlying cartilage health and potential therapeutic interventions.
Overall, the Human PIICP CLIA Kit is a valuable tool for researchers studying musculoskeletal conditions and seeking to understand the role of collagen synthesis in cartilage health and disease progression. Its high specificity and sensitivity make it a reliable choice for accurate detection of PIICP levels in clinical and research samples.
Product Name:
Human PIICP (Procollagen II C-Terminal ProPeptide) CLIA Kit
SKU:
HUES00583
Target:
Human PIICP (Procollagen II C-Terminal ProPeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PⅡCP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅡCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅡCP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PⅡCP. You can calculate the concentration of Human PⅡCP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
101-116
88-99
Average (%)
109
107
93
1:4
Range (%)
102-115
98-114
91-105
Average (%)
108
107
96
1:8
Range (%)
103-119
102-116
93-106
Average (%)
110
108
99
1:16
Range (%)
101-114
87-99
84-97
Average (%)
106
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.98
129.75
483.5
55.4
124.98
498.77
Standard deviation
4.47
14.93
31.14
6.73
13.09
55.61
C V (%)
8.44
11.51
6.44
12.15
10.47
11.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PⅡCP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PⅡCP in samples. No significant cross-reactivity or interference between Human PⅡCP and analogues was observed.
