Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) ELISA Kit
The Human PIGR (Polymeric Immunoglobulin Receptor) ELISA Kit is designed for the precise detection of PIGR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PIGR is a vital protein involved in mucosal immunity, being responsible for transporting immunoglobulin A (IgA) across mucosal surfaces. It plays a critical role in protecting the body against infections and inflammatory diseases.
Monitoring PIGR levels can provide valuable insights into the immune response and mucosal defense mechanisms.This ELISA kit from AssayGenie provides researchers with a reliable tool for studying PIGR biology and its implications in various diseases and immune responses. The kit's high performance and ease of use make it a valuable asset for laboratories conducting cutting-edge research in immunology and mucosal biology.
Product Name:
Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) ELISA Kit
SKU:
HUES02301
Target:
Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIGR/SC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIGR/SC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIGR/SC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIGR/SC. You can calculate the concentration of Human PIGR/SC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-105
98-115
Average (%)
96
96
106
1:4
Range (%)
88-100
79-91
84-96
Average (%)
95
86
91
1:8
Range (%)
93-106
80-91
86-101
Average (%)
99
86
93
1:16
Range (%)
89-103
84-98
90-101
Average (%)
97
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
91-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.27
252.56
732.1
102.39
271.44
758.06
Standard deviation
6
10.58
37.56
5.53
12.46
28.12
C V (%)
5.98
4.19
5.13
5.4
4.59
3.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PIGR/SC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PIGR/SC in samples. No significant cross-reactivity or interference between Human PIGR/SC and analogues was observed.
